Biotechnology and Its Applications (PYQs)

Q1. Transgenic plants are the ones:
Correct Answer: (a)
Transgenic plants contain recombinant foreign gene fragments stably integrated into their genomes via transformation and tissue culture regeneration.
Q2. Silencing of mRNA has been used in producing transgenic plants resistant to:
Correct Answer: (b)
mRNA silencing via RNAi is employed to develop transgenic crop plants immune to pathogenic soil nematodes.
Q3. Exploration of molecular, genetic and species level diversity for novel products of economic importance is known as:
Correct Answer: (a)
Bioprospecting refers to systematically searching for, collecting, and exploring natural genetic and molecular materials for commercially valuable products.
Q4. What is true about Bt toxin?
Correct Answer: (d)
Bt toxin is synthesized as an inactive crystalline protoxin inside the bacterium and becomes activated only after ingestion due to the alkaline pH of the insect midgut.
Q5. Match the following columns and select the correct option.
Column-IColumn-II
(a) Bt cotton(i) Gene therapy
(b) Adenosine deaminase deficiency(ii) Cellular defence
(c) RNAi(iii) Detection of HIV infection
(d) PCR(iv) Bacillus thuringiensis
Correct Answer: (d)
Bt cotton matches Bacillus thuringiensis; ADA deficiency matches Gene therapy; RNAi matches Cellular defence; PCR matches Detection of HIV infection.
Q6. Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?
Correct Answer: (c)
The genetic code is nearly universal across organisms; hence a specific codon specifies the exact same amino acid in bacteria as it does in humans.
Q7. The genetically-modified (GM) brinjal in India has been developed for:
Correct Answer: (a)
Bt brinjal was developed by inserting the cry1Ac gene from Bacillus thuringiensis to impart resistance against the Fruit and Shoot Borer.
Q8. Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
Correct Answer: (b)
Biopiracy denotes patenting and exploiting native bioresources without authorization or fair compensatory payment to traditional owners.
Q9. Which of the following statements is not correct?
Correct Answer: (b)
Functional mature human insulin consists of two polypeptide chains (A and B) linked together by disulfide bonds, not hydrogen bonds.
Q10. Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of:
Correct Answer: (c)
Golden rice biosynthesizes beta-carotene, which is converted into Vitamin A in the human metabolic pathway.
Q11. Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?
Correct Answer: (c)
Golden rice is enriched with beta-carotene (provitamin A) specifically engineered to combat night blindness and xerophthalmia caused by Vitamin A deficiency.
Q12. The first clinical gene therapy was given for treating
Correct Answer: (d)
The first clinical gene therapy was administered in 1990 to a 4-year-old girl suffering from Adenosine Deaminase (ADA) deficiency.
Q13. What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?
Correct Answer: (d)
The inactive protoxin ingested by the insect is solubilized and converted into an active toxin due to the alkaline pH of the insect's midgut.
Q14. Read the following four statements (A-D) about certain mistakes in two of them:
(A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
(B) Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C) Downstream processing is one of the steps of r-DNA technology.
(D) Disarmed pathogen vectors are also used in transfer of r-DNA into the host.
Which are the two statements having mistakes?
Correct Answer: (d)
Statement A is wrong because Rosie was a transgenic cow (not buffalo). Statement B is wrong because restriction enzymes cut DNA at specific recognition sites, whereas enzymes like cellulase/chitinase/protease are used in isolation.
Q15. The name of Norman Borlaug is associated with:
Correct Answer: (b)
Dr. Norman Borlaug, known as the 'Father of the Green Revolution', developed high-yielding semi-dwarf wheat varieties that drastically increased global agricultural production.
Q16. Maximum number of existing transgenic animals is of:
Correct Answer: (b)
Over 95% of all existing transgenic laboratory animal models produced for biomedical research are mice.
Q17. The crops engineered for glyphosate are resistant/ tolerant to:
Correct Answer: (c)
Glyphosate is an active ingredient in many broad-spectrum herbicides. Crops engineered for glyphosate tolerance can survive application of these weed-killers.
Q18. Exploitation of bioresources of a nation by multinational companies without authorisation from the concerned country is referred to as
Correct Answer: (c)
Biopiracy is the unauthorized commercial exploitation of biological resources and indigenous knowledge of target developing nations.
Q19. In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to:
Correct Answer: (c)
The protoxin dissolves and gets proteolytically cleaved into its active form within the insect gut due to its high alkaline pH environment.
Q20. RNA interference involves
Correct Answer: (c)
RNAi is a cellular defense mechanism where sequence-specific post-transcriptional gene silencing occurs via double-stranded complementary RNA.
Q21. Which part of the tobacco plant is infected by Meloidogyne incognita?
Correct Answer: (d)
Meloidogyne incognita is a nematode (roundworm) that infects the roots of tobacco plants, causing root-knot disease which significantly reduces crop yield.
Q22. Which one of the following vectors is used to replace the defective gene in gene therapy?
Correct Answer: (b)
Viral vectors like Adenovirus and Retrovirus are commonly modified to deliver functional replacement genes into host human cells during gene therapy.
Q23. A 'new' variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to
Correct Answer: (c)
In 1997, a US firm obtained a patent on Basmati rice lines derived from traditional Indian rice varieties, sparking major biopiracy debate.
Q24. Genetically engineered bacteria have been successfully used in the commercial production of
Correct Answer: (a)
Recombinant E. coli cells containing cloned synthetic DNA fragments synthesize the A and B chains of human insulin.
Q25. Main objective of production/use of herbicide resistant GM crops is to
Correct Answer: (a)
Herbicide-resistant crops allow farmers to spray non-selective herbicides to wipe out weeds cleanly without damaging the main crop or requiring extensive manual weeding.
Q26. Cry endotoxins obtained from Bacillus thuringiensis are effective against
Correct Answer: (d)
Cry endotoxins encoded by cry1Ac and cry2Ab genes are highly effective in killing lepidopteran pests like cotton bollworms.
Q27. Ultrasound of how much frequency is beamed into human body for sonography?
Correct Answer: (b)
Diagnostic medical ultrasound operates using high-frequency sound waves in the range of 1 to 15 MHz.
Q28. What is true for monoclonal antibodies?
Correct Answer: (a)
Monoclonal antibodies are homogeneous antibody molecules produced by single B-cell clones (via hybridoma technology) specific to a single epitope of an antigen.
Q29. A transgenic food crop which may help in solving the problem of night blindness in developing countries is
Correct Answer: (d)
Golden rice contains elevated levels of provitamin A (beta-carotene) to address nutritional Vitamin A deficiencies and prevent night blindness.
Q30. Genetic engineering has been successfully used for producing:
Correct Answer: (a)
Transgenic mice were engineered and successfully used to test the batch safety of polio vaccines before human trials.
Q31. Continuous addition of sugars in 'fed batch' fermentation is done to:
Correct Answer: (b)
Fed-batch fermentation allows sustained nutrient supply at optimal rates, enhancing biomass concentration and yield of secondary metabolites like antibiotics.
Q32. In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is
Correct Answer: (c)
The Genetic Engineering Appraisal Committee (GEAC) under the MoEFCC regulates environmental releases and public approvals of GM organisms in India.
Q33. Consider the following statements about biomedical technologies:
1. During open heart surgery blood is circulated in the heart-lung machine
2. Blockage in coronary arteries is removed by angiography
3. Computerised axial tomography (CAT) shows detailed internal structure as seen in a section of body
4. X-ray provides clear and detailed images of organs like prostate glands and lungs
Which two of the above statements are correct?
Correct Answer: (c)
Statements 1 and 3 are correct. Angiography is a diagnostic visualization method (not removal), and standard soft tissues are poorly detailed by conventional X-rays.
Q34. Two microbes found to be very useful in genetic engineering are
Correct Answer: (d)
Escherichia coli is the primary bacterial host for gene cloning, and Agrobacterium tumefaciens serves as the premier vector for plant gene transfer.
Q35. The most common substrate used in distilleries for the production of ethanol is
Correct Answer: (d)
Molasses, a byproduct of the sugar refining industry containing concentrated sucrose and fermentable sugars, is the primary substrate for industrial ethanol fermentation.
Q36. Which of the following is true for Golden rice?
Correct Answer: (a)
Golden rice is a genetically modified variety engineered with beta-carotene (provitamin A) biosynthesis genes derived from daffodil (Narcissus pseudonarcissus) and bacteria.
Q37. Which of the following Bt crops is being grown in India by the farmers?
Correct Answer: (a)
Bt cotton is the major commercially approved GM crop widely grown by farmers across India for insect pest management.
Q38. Bacillus thuringiensis forms protein crystals which contain insecticidal protein that
Correct Answer: (a)
Activated Bt endotoxins bind to the surface receptors of midgut epithelial cells, forming pores that cause swelling, cell lysis, and death of the insect.
Q39. Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
Correct Answer: (a)
Nematode-specific genes introduced into the host produce both sense and anti-sense RNA, forming double-stranded RNA (dsRNA) that initiates RNAi.
Q40. The first human hormone produced by recombinant DNA technology is:
Correct Answer: (a)
Human insulin (Humulin), developed commercially by Eli Lilly in 1983, was the first therapeutic hormone produced using recombinant DNA technology in E. coli.
Q41. Bacillus thuringiensis (Bt) strains have been used for designing novel:
Correct Answer: (d)
Genes from Bacillus thuringiensis are isolated and transferred into crop genomes to produce transgenic bioinsecticidal plants like Bt cotton and Bt corn.
Q42. The bacterium Bacillus thuringiensis is widely used in contemporary biology as
Correct Answer: (a)
Bacillus thuringiensis produces delta-endotoxin protein crystals that make it a powerful biological insecticide against targeted plant pests.
Q43. Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?
Correct Answer: (b)
Genetic Engineering Approval (now Appraisal) Committee (GEAC) evaluates GM research safety and environmental release approvals in India.
Q44. Consider the following statements (A-D) about organic farming:
(A) Utilizes genetically modified crops like Bt cotton
(B) Uses only naturally produced inputs like compost
(C) Does not use pesticides and urea
(D) Produces vegetables rich in vitamins and minerals
Which of the above statements are correct?
Correct Answer: (c)
Organic farming strictly avoids artificial synthetic inputs like chemical fertilizers (urea), synthetic pesticides, and GM crops, relying instead on natural compost and biological control.
Q45. The process of RNA interference has been used in the development of plants resistant to
Correct Answer: (a)
RNA interference was famously utilized to protect tobacco plants against the parasitic root-knot nematode Meloidogyne incognita.
Q46. Golden rice is a transgenic crop of the future with the following improved trait:
Correct Answer: (d)
The key trait of Golden rice is biofortification with high pro-Vitamin A (beta-carotene) levels.
Q47. Pollen tablets are available in the market for:
Correct Answer: (c)
Pollen grains are extremely rich in proteins, carbohydrates, and essential nutrients, so pollen tablets/syrups are commercialized as dietary food supplements.
Q48. One of the most important reasons why wild plants should thrive is that they are good sources of
Correct Answer: (c)
Wild varieties hold valuable evolutionary gene pools, providing essential resistance genes against pests, diseases, and environmental stresses for crop breeding.
Q49. In RNAi, the genes are silenced using :
Correct Answer: (b)
RNA interference (RNAi) involves the silencing of a specific mRNA due to a complementary double-stranded RNA (dsRNA) molecule that binds to and prevents translation of the target mRNA.
Q50. Some of the characteristics of Bt cotton are:
Correct Answer: (d)
Bt cotton expresses cry1Ac and cry2Ab genes, conferring specific resistance against lepidopteran pests like cotton bollworms, ensuring higher crop productivity.
Q51. Maximum application of animal cell culture technology today is in the production of
Correct Answer: (d)
Large-scale animal cell culture is extensively deployed worldwide primarily for the commercial production of viral vaccines.

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