Photosynthesis in Higher Plants (MCQs)

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Q1. At which wavelength cyclic phosphorylation occurs?
Correct Answer: (b)
Cyclic photophosphorylation only involves PSI, which can be activated by light of wavelengths longer than 680 nm where PSII (required for non-cyclic) is inactive.
Q2. With regard to the RuBisCO, which of the following statement(s) is/are correct?
(1) It has a similar affinity for both CO2 and O2.
(2) It is the most abundant protein present on earth.
(3) The relative concentration of O2 and CO2 determines which of the two will bind to the enzyme.
Correct Answer: (b)
RuBisCO has a much higher affinity for CO2 than O2. It is the most abundant protein, and binding depends on relative gas concentrations.
Q3. Which one of the following statement is incorrect in relation to photorespiration?
Correct Answer: (c)
Photorespiration does not produce ATP or NADPH; instead, it consumes ATP and releases CO2, making it a wasteful process.
Q4. Identify the correct statements for ATP synthase:
(i) This enzyme consists of two parts: hydrophobic membrane bound portion called F0 & a portion that sticks out into stroma called F1.
(ii) F0 appears to form a channel across the membrane through which proton can pass.
(iii) The conformational change in the F1 portion of the complex synthesizes ATP.
(iv) The proton motive force that drives the synthesis of ATP is associated with this enzyme.
Correct Answer: (b)
Statement (iii) is slightly incomplete; it's the passage of protons through the channel and the resulting rotation/conformational change in the F1 head that leads to ATP synthesis from ADP and Pi.
Q5. The principle of limiting factors was proposed by
Correct Answer: (a)
F.F. Blackman proposed the Law of Limiting Factors in 1905, stating that if a process is influenced by multiple factors, its rate is limited by the factor nearest to its minimum value.
Q6. Dark reaction in photosynthesis is called so because:
Correct Answer: (b)
The dark reaction is called so because it is not directly light-driven, although it depends on the ATP and NADPH produced during the light reaction.
Q7. Which of the following characteristics out of I, II and III are exhibited by C4 plants?
I. Kranz anatomy.
II. The product of photosynthesis is oxalo acetic acid.
III. Both PEP carboxylase and ribulose-bi phosphate carboxylase act as carboxylating enzyme.
Correct Answer: (d)
C4 plants have Kranz anatomy (I), produce OAA as the first product (II), and utilize both PEPcase (in mesophyll) and RuBisCO (in bundle sheath) for carboxylation (III).
Q8. Which of the following statement is incorrect regarding pigments?
Correct Answer: (b)
Chlorophyll a is the chief pigment of photosynthesis. Chlorophyll b is an accessory pigment.
Q9. The correct sequence of flow of electrons in the light reaction is:
Correct Answer: (a)
Electrons flow from water to PSII, then to Plastoquinone, through the Cytochrome b6f complex, to PSI, and finally to Ferredoxin/NADP+.
Q10. In the leaves of C4 plants, malic acid formation during CO2 fixation occurs in the cells of
Correct Answer: (d)
Initial CO2 fixation into C4 acids like malic or aspartic acid takes place in the mesophyll cells of C4 leaves.
Q11. The given figure shows the diagrammatic representation of a section of chloroplast. Few parts are marked as A, B, C, D & E.

Combination of which parts is responsible for trapping the light energy & also the synthesis of ATP and NADPH?
Correct Answer: (a)
A (Grana), B (Stroma lamellae), and C (Thylakoids) represent the membrane system of the chloroplast where the light reaction occurs.
Q12. Reduction of NADP+ into NADPH during light reaction occurs in stroma because:
Correct Answer: (a)
The NADP reductase enzyme is located on the stromal side of the thylakoid membrane, so the final reduction happens in the stroma.
Q13. Assume the thylakoid membrane within a chloroplast is punctured so that there is no separation between lumen & stroma of thylakoid. Which of these process would be most affected?
Correct Answer: (b)
Without a membrane barrier, a proton gradient cannot be maintained. Since the gradient drives ATP synthesis via chemiosmosis, ATP production will fail.
Q14. How can we separate leaf pigments of any green plant?
Correct Answer: (b)
Paper chromatography is a standard technique used to separate and identify the different pigments present in a leaf (chlorophyll a, chlorophyll b, xanthophylls, and carotenoids).
Q15. Match the parts given in column I with the events given in column II and choose the correct combination from the options given below.
Column-IColumn-II
A. Grana of chloroplastI. Kreb's cycle
B. Stroma of chloroplastII. Light reaction
C. CytoplasmIII. Dark reaction
D. Mitochondrial matrixIV. Glycolysis
Correct Answer: (d)
Grana (Light reaction), Stroma (Dark reaction), Cytoplasm (Glycolysis), Mitochondrial Matrix (Kreb's cycle).
Q16. Water soluble pigments found in plant cell vacuoles are:
Correct Answer: (d)
Anthocyanins are water-soluble pigments (providing red/purple/blue colors) stored in the vacuole, unlike chlorophylls and carotenoids which are lipid-soluble and in plastids.
Q17. Which of the following statement(s) is/are incorrect ?
(a) H2S, not H2O, is involved in photosynthesis of purple sulphur bacteria.
(b) Light and dark reactions are stopped in the absence of light.
(c) Calvin cycle occurs in the grana of chloroplast.
Correct Answer: (d)
The dark reaction occurs in the stroma, not grana. Both (b) and (c) are incorrect in this biological context.
Q18. Chromatophores take part in:
Correct Answer: (d)
In prokaryotes, chromatophores are pigment-containing internal membrane structures that perform photosynthesis.
Q19. The primary CO2 acceptor in C4 plant is
Correct Answer: (b)
The CO2 acceptor in C4 plants is Phosphoenol pyruvate (PEP), a 3-carbon molecule located in the mesophyll cells.
Q20. In a crop field a weedicide is used to remove weeds in order to increase the yield. But the effect of this weedicide is that, it blocks electron transport from photosystem II to photosystem I. This will result in
Correct Answer: (d)
Blocking electron flow stops both the establishment of the proton gradient (no ATP) and the arrival of electrons at PSI (no NADPH).
Q21. The reaction that is responsible for the primary fixation of CO2 is catalysed by:
Correct Answer: (c)
Depending on the plant type (C3 or C4), the primary fixation is done by either RuBisCO or PEPcase.
Q22. In non-cyclic photophosphorylation, there is photolysis of 12 water molecules. How many H+ are formed ?
Correct Answer: (a)
Each molecule of water (H2O) split releases 2 protons (H+). Therefore, 12 molecules of water release 24 protons.
Q23. Which one represents the correct empirical equation of photosynthesis?
Correct Answer: (d)
The balanced chemical equation for photosynthesis highlights that 12 molecules of water are required to produce 6 molecules of oxygen and 1 molecule of glucose, with 6 molecules of water being re-formed.
Q24. Chemosynthetic bacteria obtain energy from:
Correct Answer: (d)
Chemosynthetic bacteria oxidize various inorganic substances (nitrates, nitrites, ammonia) to obtain energy for their food production.
Q25. The given figure of calvin cycle shows the carbon assimilation in C3 plants. Choose the correct labelling of the carbohydrate molecule (Marked as I, II and III) involved in the Calvin cycle.

(I)(II)(III)
(a)RuBPTriose phosphatePGA
(b)PGARuBPTriose phosphate
(c)PGATriose phosphateRuBP
(d)RuBPPGATriose phosphate
Correct Answer: (b)
In the cycle: Fixation product is PGA (I). The 5-carbon acceptor is RuBP (II). Reduction leads to Triose phosphate (III).
Q26. ADP is phosphorylated and NADP is reduced, this happens during.
Correct Answer: (b)
The production of ATP (phosphorylation) and NADPH (reduction) are the primary results of the light-dependent reactions.
Q27. Cooperation of the two photosystems of the chloroplast is required for:
Correct Answer: (b)
Non-cyclic electron transport (Z-scheme) requires both PSII (to split water and start electron flow) and PSI (to ultimately reduce NADP+ to NADPH).
Q28. With regard to the proton gradient associated with chloroplasts, consider the following statements:
(1) Within the chloroplast, protons in the stroma increase in number than the lumen.
(2) The gradient is broken down due to the movement of electrons across the membrane.
Correct Answer: (d)
Both are incorrect. Protons increase in the lumen, and the gradient is broken down by the movement of protons through ATP synthase.
Q29. Oxygen is not produced during photosynthesis by:
Correct Answer: (a)
Anoxygenic photosynthetic bacteria like green sulphur bacteria use H2S as a hydrogen donor, releasing sulphur instead of oxygen.
Q30. The given figure shows the diagrammatic representation of the Hatch & Slack pathway few labelling are marked as P, Q and R.


Which of the following option shows the correct labeling of P, Q, and R?
Correct Answer: (b)
In C4 plants: Atmospheric CO2 is fixed (P) into a C4 acid (Q). After transport and decarboxylation, the residue returns to complete regeneration (R).
Q31. In the light reaction of photosynthesis electrons are picked up by an electron acceptor which passes them to an electron transport system consisting of:
Correct Answer: (b)
The electron transport chain in chloroplasts includes a series of carriers, notably the cytochromes.
Q32. Which of the following is not concern with cyclic photophosphorylation?
Correct Answer: (a)
Oxygen is only liberated during non-cyclic photophosphorylation because that is where the water-splitting PSII is active.
Q33. Given below is the pathway (Z-scheme) of light reaction. Identify the blanks indicated by A, B, C and D.
Correct Answer: (d)
The Z-scheme starts with PSII (A), which passes electrons to a primary acceptor (B), through an ETC to PSI (C), ultimately reducing NADP+ to NADPH (D).
Q34. Which of the following plant species have highest photosynthetic yield?
Correct Answer: (c)
C4 plants are more productive because they have a mechanism to concentrate CO2 and avoid wasteful photorespiration.
Q35. Which of the following statement(s) is/are correct about RuBisCO?
Correct Answer: (d)
RuBisCO (Ribulose-1,5-bisphosphate carboxylase-oxygenase) is a dual-function enzyme that is the most widespread and abundant protein globally.
Q36. Assume a thylakoid is somehow punctured so that the interior of the thylakoid is no longer separated from the stroma. This damage will have the most direct effect on which of the following processes?
Correct Answer: (d)
ATP synthesis depends on the maintenance of a high proton concentration in the thylakoid lumen relative to the stroma. Puncturing the membrane allows protons to leak out, destroying the gradient and stopping ATP production.
Q37. An energy diagram for the transfer of electrons in the light reactions of photosynthesis in plants is:
Correct Answer: (c)
The specific zigzag shape of the energy level diagram of electron transport in the light reaction is called the Z-scheme.
Q38. Chlorophyll is suited for the capture of light energy because:
Correct Answer: (d)
Chlorophyll absorbs specific light energy, which excites its electrons, and its hydrophobic tail keeps it anchored in the thylakoid membrane.
Q39. Which light range is most effective in photosynthesis?
Correct Answer: (c)
While blue light is absorbed, red light is the most efficient wavelength for driving photosynthesis per unit of absorbed energy.
Q40. The function of water in photosynthesis is to:
Correct Answer: (b)
Water acts as the ultimate electron donor, replacing electrons lost from photosystems during light capture.
Q41. According to Blackman’s law of limiting factor, at any given time, photosynthesis can be limited by:
Correct Answer: (d)
The process is always limited by the single specific factor that is in the shortest supply relative to requirements at that moment.
Q42. Every CO2 molecule entering the Calvin cycle needs:
Correct Answer: (a)
To fix one CO2 molecule, the cycle requires 3 ATPs and 2 NADPHs.
Q43. The total requirement of ATP & NADPH for each molecule of CO2 fixed & reduced in photosynthesis in the Calvin cycle is
Correct Answer: (c)
Per CO2 molecule fixed, the Calvin cycle consumes 3 molecules of ATP (2 in reduction, 1 in regeneration) and 2 molecules of NADPH (in reduction).
Q44. In non-cyclic reactions of photosynthesis, electrons from chlorophyll molecules in photosystem-I are used in the formation of NADPH. What is the source of such electrons?
Correct Answer: (d)
In the Z-scheme, electrons lost by PSI are replaced by electrons originating from water splitting at PSII and passed down the ETC.
Q45. Chloroplasts are disrupted and the stroma separated from the lamellae. The isolated stroma will fix CO2 if it is supplied with:
Correct Answer: (d)
Stroma contains the enzymes for the dark reaction (Calvin cycle). If provided with the products of light reaction (ATP and NADPH), it can perform carbon fixation in vitro.
Q46. Which is the primary CO2 fixation product in C4 plant?
Correct Answer: (b)
C4 plants are named so because the first stable product formed by CO2 fixation is the 4-carbon Oxaloacetic acid.
Q47. Which of the following statements (i-iv) regarding “Splitting of water” is/are correct?
(i) It is photolysis of water which provides H+ ions for synthesis of NADPH.
(ii) It provides electrons for photophosphorylation & activation of NADP+.
(iii) O2 is evolved during this process.
(iv) It replenishes O2 consumed by living beings and combustion.
Correct Answer: (d)
All given statements correctly describe the role and outcomes of the photolysis of water in photosynthesis.
Q48. During light reaction in photosynthesis the following are formed:
Correct Answer: (c)
The primary products are ATP, NADPH (the hydrogen donor), and oxygen (released as a byproduct).
Q49. The correct sequence of Calvin cycle is:
Correct Answer: (d)
The Calvin cycle proceeds through three main stages: Carboxylation (fixation), Reduction (using ATP/NADPH), and Regeneration (of the CO2 acceptor RuBP).
Q50. In a chloroplast, the highest number of protons are found in
Correct Answer: (b)
During light reactions, protons are actively pumped into the thylakoid lumen, making it the area with the highest concentration and lowest pH.
Q51. Which of the following does participate in the process of photosynthesis?
Correct Answer: (d)
All these groups of algae contain photosynthetic pigments (like chlorophyll a) and perform photosynthesis.
Q52. What is/are the function(s) of accessory pigments?
Correct Answer: (d)
Accessory pigments (chlorophyll b, xanthophylls, and carotenoids) broaden the absorption spectrum and protect the primary pigment, chlorophyll a, from damage by intense light.
Q53. A student sets up an experiment on photosynthesis as follows: He takes soda water in a glass tumbler and add chlorophyll extracts into the contents and keeps the tumbler exposed to sunlight hoping that he has provided necessary ingredients for photosynthesis to proceed (viz., CO2, H2O, chlorophyll and light). What do you think what will happen after, few hours of exposure of light?
Correct Answer: (d)
Photosynthesis is not just a chemical mixture; it requires a highly organized membrane system (intact chloroplasts) to create the necessary proton gradients and light harvesting structures.
Q54. Two groups of isolated thylakoids are placed in an acidic bathing solution so that H+ diffuses into the thylakoids. They are then transferred to a basic bathing solution, and one group is placed in the light, while the other group is kept in the dark. Select the choice given below that describes what you expect each group of thylakoids to produce.
In LightIn Dark
(a)ATP onlyNothing
(b)ATP, O2ATP only
(c)ATP, O2, glucoseATP, O2
(d)ATP, O2O2
Correct Answer: (b)
The pre-established pH gradient (acidic inside, basic outside) allows for ATP synthesis via chemiosmosis even in the dark. In light, the thylakoids will also split water and produce O2.
Q55. In maximum plants, stomata open during day and closed in night. Its exception is:
Correct Answer: (a)
CAM plants (like cacti) open their stomata at night to fix CO2 as organic acids and keep them closed during the day to minimize water loss.
Q56. The enzyme that is not found in a C3 plant is:
Correct Answer: (b)
C3 plants fix CO2 only through the Calvin cycle using RuBisCO; they do not have the specialized PEPcase mechanism of C4 plants.
Q57. Which was the first CO2 fixation product formed, in the Calvin experiment, using radioactive labelled 14C in green algale?
Correct Answer: (b)
Melvin Calvin discovered that the first stable product after carbon fixation in the C3 cycle is 3-phosphoglyceric acid (3-PGA), a 3-carbon compound.
Q58. Which of the following is incorrectly matched?
Correct Answer: (b)
P700 is the reaction center of Photosystem I. PSII uses P680.
Q59. The given figure shows the graph of light intensity (on x-axis) on the rate of photosynthesis (on y-axis). Few points are marked as A, B, C, D and E.

Match the marked alphabets given in column I with these correct interpretation given in column II.
Column - IColumn - II
A. Limiting factor in region AI. Some factor other than light intensity is becoming the limiting factor
B. B representsII. Light is no longer limiting factor
C. C representsIII. Light intensity
D D representsIV. Maximum rate of photosynthesis
E. E representsV. Saturation point for light intensity
Correct Answer: (b)
In region A, light intensity (III) is limiting. B represents the transition where other factors (I) take over. C is where light is no longer limiting (II). D is the saturation point (V) and E is the max rate (IV).
Q60. The pigment molecules responsible for photosynthesis are located in the:
Correct Answer: (c)
The light-harvesting complexes and reaction centers are embedded within the thylakoid membranes of the chloroplast.
Q61. Bundle sheath cells:
Correct Answer: (a)
Bundle sheath cells contain high concentrations of RuBisCO to perform the Calvin cycle, but they lack PEPcase.
Q62. Which of the following statements given below is incorrect ?
Correct Answer: (d)
Water stress causes stomata to close, which reduces CO2 availability and lowers the rate of photosynthesis.
Q63. Why, at higher light intensities, gradually photosynthesis rate does not show further increase?
Correct Answer: (d)
Once light is no longer the limiting factor, the rate is restricted by other variables such as CO2 concentration or temperature.
Q64. The diagram given below shows ATP synthesis through chemiosmosis.

Which option shows the correct labelling of A, B, C and D in the diagram ?
Correct Answer: (c)
A (F1 headpiece), B (Thylakoid membrane), C (Photosystem II - where water splitting occurs), D (Photosystem I).
Q65. In a plant cell, which of the following pigments participates directly in the light reactions of photosynthesis?
Correct Answer: (a)
Chlorophyll a is the primary pigment that acts as the reaction center where the light energy is converted into chemical energy.
Q66. During photosynthesis when PGA is changed into phosphoglyceraldehyde, which of the following reaction occur?
Correct Answer: (b)
The conversion of 3-PGA to triose phosphate (G-3-P) is a reduction reaction requiring NADPH and ATP.
Q67. The oxygenation activity of RuBisCo enzyme in photorespiration leads to the formation of
Correct Answer: (a)
RuBisCO oxygenase activity splits RuBP into 1 molecule of 3-PGA (3-C) and 1 molecule of 2-phosphoglycolate (2-C).
Q68. A process that creates an important difference between C3 & C4 plants is called____________.
Correct Answer: (c)
The ability to minimize photorespiration is the primary physiological distinction that makes C4 plants more efficient than C3 plants.
Q69. During monsoon, the rice crop of eastern states of India shows lesser yield due to limiting factor of
Correct Answer: (b)
During the monsoon, cloudy skies reduce the amount of sunlight, making light the limiting factor for crop productivity.
Q70. Under water stress, the rate of photosynthesis declines because of
Correct Answer: (c)
Water stress triggers stomatal closure (less CO2) and causes wilting (less surface area), both of which inhibit photosynthesis.
Q71. Correct equation that represents the photolysis of water is
Correct Answer: (a)
The splitting of water releases electrons, protons, and molecular oxygen: 2H2O → 4H+ + O2 + 4e.
Q72. In C3 plant, when O2 concentration is more, the O2 binds to RuBisCo and RuBP gets changed to
Correct Answer: (d)
In the oxygenase reaction of RuBisCO, RuBP reacts with O2 to form one molecule of 3-PGA and one molecule of 2-phosphoglycolate.
Q73. Photosynthesis is a/an:
Correct Answer: (a)
Photosynthesis is a physico-chemical process by which plants use light energy to drive the synthesis of organic compounds.
Q74. When CO2 is added to PEP, the first stable product synthesised is:
Correct Answer: (d)
In C4 plants, the fixation of CO2 by PEP results in the 4-carbon oxaloacetate.
Q75. A plant in your garden avoids photorespiratory losses, has improved water use efficiency, shows high rates of photosynthesis at high temperatures and has improved efficiency of nitrogen utilisation. In which of the following physiological groups would you assign this plant?
Correct Answer: (b)
These characteristics are the defining physiological advantages of C4 plants over C3 plants.
Q76. The structures that are formed by stacking of organized flattened membranous sacs in the chloroplasts are :
Correct Answer: (a)
Stacks of individual thylakoid sacs are collectively called grana.
Q77. The light-driven synthesis of ATP & NADPH, provides energy and reducing power for the:
Correct Answer: (d)
ATP and NADPH produced in light reactions are the 'assimilatory power' used in the stroma to fix carbon dioxide into carbohydrates.
Q78. Splitting of water is associated with:
Correct Answer: (d)
The water-splitting complex is physically located on the inner side of the thylakoid membrane.
Q79. Which one of the following correctly identifies X and Y and and their functions in the given figure of chloroplast ?
Correct Answer: (a)
In the chloroplast, grana (X) are the site of light reactions (including water splitting), and the stroma (Y) is the site of the dark reactions (carbon fixation).
Q80. Cyclic and non-cyclic flow of e is used in plants to:
Correct Answer: (d)
Non-cyclic flow produces both ATP and NADPH, while cyclic flow produces only extra ATP, allowing the cell to maintain the correct ratio needed for carbon fixation.
Q81. The light harvesting complex (LHC) is made up of
Correct Answer: (c)
The LHC consists of hundreds of pigment molecules (chlorophyll b, xanthophylls, carotenoids) associated with specific proteins.
Q82. In C4 (sugarcane plant) plant, 14CO2 is fixed in malic acid. Which of the following enzymes is used to fix CO2?
Correct Answer: (c)
In C4 plants, the initial fixation of atmospheric CO2 is performed by PEP carboxylase (phosphoenol pyruvic acid carboxylase).
Q83. The reactions of Calvin cycle not directly dependent on light, but they usually do not occur at night. Why?
Correct Answer: (d)
The Calvin cycle requires a continuous supply of ATP and NADPH, which are only generated during the light-dependent phase.
Q84. Photophosphorylation in chloroplast is most similar to the:
Correct Answer: (b)
Both processes utilize an electron transport chain to create a proton gradient that drives ATP synthesis through ATP synthase.
Q85. Which one of the following is incorrect about the activities associated with PS-I and PS-II in non-cyclic photophosphorylation?
Correct Answer: (d)
In non-cyclic electron transport, NADPH is produced on the stroma side of the membrane associated with the electrons coming from Photosystem I.
Q86. Which metal ion is a constituent of chlorophyll?
Correct Answer: (c)
Chlorophyll has a magnesium atom at the center of its porphyrin ring structure.
Q87. Match the column-1 with column II and choose the correct answer using the codes given below.
Column-IColumn-II
A. Emerson effectI. C4 cycle
B. Hill reactionII. Photolysis
C. Calvin cycleIII. C3 cycle
D. Hatch & Slack cycleIV. Photosystem I & II
Correct Answer: (d)
Emerson effect (evidence for two photosystems), Hill reaction (photolysis of water), Calvin cycle (C3 pathway), Hatch & Slack (C4 pathway).
Q88. Which of the following pair is mismatched?
Correct Answer: (d)
The dark reaction (Calvin cycle) takes place in the stroma, not the grana.
Q89. The oxygen evolved during photosynthesis comes from water molecules. Which one of the following pairs of elements is involved in this reaction?
Correct Answer: (d)
Manganese and Chloride ions are essential cofactors for the water-splitting enzyme system in PSII.
Q90. Match column-I with column-II and choose the correct combination from the options given below.
Column-IColumn-II
A. Oxygen evolving complex ferric oxalateI. Pigments
B. Proton gradient concentrationII. High oxygen
C. Absorb light at specific wavelengths.III. ATP synthesis
D. PhotorespirationIV. Photolysis of water
Correct Answer: (a)
A-IV (OEC does photolysis), B-III (gradient drives ATP synthesis), C-I (Pigments absorb light), D-II (Photorespiration occurs at high O2 levels).
Q91. In Calvin cycle, RuBisCO incorporates CO2 into ribulose, 1, 5-bisphosphate which rapidly splits into
Correct Answer: (a)
The reaction of 5C-RuBP and 1C-CO2 produces an unstable 6C intermediate that immediately breaks into two 3C molecules of 3-PGA.
Q92. Consider the following statements:
(1) In Blackman’s Law, if a chemical process is affected by more than one factor, then its rate will be determined by the factor which is nearest to its minimal value.
(2) For photosynthesis, light is rarely a limiting factor in nature.
Correct Answer: (c)
Both statements are correct. Blackman's law relies on the limiting (minimal) factor, and CO2 is usually the limiting factor in nature, not light.
Q93. Which one of the following statement correctly describes the cyclic photophosphorylation?
Correct Answer: (d)
In cyclic flow, electrons expelled from the reaction center of PSI are passed through carriers and eventually returned back to the same reaction center.
Q94. Energy required for ATP synthesis in PS II comes from
Correct Answer: (a)
The energy for ATP synthesis is derived from the potential energy stored in the transmembrane proton gradient.
Q95. During photosynthesis, electrons are continuously lost from the reaction centre of PSII. By which process these electrons are replaced?
Correct Answer: (b)
Electrons needed by the PSII reaction center are supplied by the electrons released during the splitting of water.
Q96. Anoxygenic photosynthesis is characteristic of:
Correct Answer: (a)
Purple non-sulphur bacteria like Rhodospirillum perform photosynthesis using hydrogen from compounds other than water, so no oxygen is evolved.
Q97. ATP synthesis, in photosynthesis involves the:
Correct Answer: (a)
ATP synthesis is coupled to the establishment of a proton (H+) gradient across the thylakoid membrane.
Q98. With regard to the electron transport during photosynthesis, consider the following statements:
(1) The movement of electrons is downhill, in terms of redox potential scale.
(2) The electrons are used up as they pass through the electron transport chain.
Correct Answer: (a)
Statement (1) is correct regarding movement between carriers. (2) is incorrect because electrons are eventually transferred to NADP+, not 'used up'.
Q99. Which among the following sentence is incorrect about light reaction?
Correct Answer: (c)
The reaction center always consists of a specific chlorophyll a molecule (P680 for PSII or P700 for PSI), not chlorophyll b.
Q100. Why are protons transported across the thylakoid membrane into the lumen?
Correct Answer: (d)
PQ (Plastoquinone) is a hydrogen carrier. As it accepts electrons on the stroma side, it also picks up protons. When it moves to the lumen side to pass electrons to the next carrier, it releases those protons into the lumen.
Q101. Study the given graph which shows the action spectrum of A. Superimposed on B spectrum of chlorophyll a. Identify A & B in the graph.

AB
(a)Rate of respirationAction spectrum
(b)Rate of respirationAbsorption
(c)Rate of photosynthesisAction spectrum
(d)Rate of photosynthesisAbsorption
Correct Answer: (c)
The graph shows that the rate of photosynthesis (A) closely follows the absorption spectrum (B) of pigments like chlorophyll a, creating the action spectrum.
Q102. The experiment material used by Van Niel, to prove that O2 comes out from water was studied on
Correct Answer: (c)
Cornelius van Niel's studies on purple and green sulphur bacteria demonstrated that photosynthesis is a light-dependent reaction where hydrogen from a suitable oxidizable compound reduces carbon dioxide to carbohydrates.
Q103. Number of carbons in the primary CO2 fixation products in C3 plant is
Correct Answer: (a)
The first stable product of the Calvin cycle (C3 cycle) is 3-phosphoglyceric acid (3-PGA), which has 3 carbons.
Q104. Which range of wavelength (in nm) is called Photosynthetically Active Radiation (PAR)?
Correct Answer: (c)
PAR covers the visible spectrum from 400 nm to 700 nm, which plants use for photosynthesis.
Q105. One of the earliest experiments on photosynthesis was done in 1770 by Joseph Priestley. He demonstrated that:
Correct Answer: (c)
Priestley's experiments with a bell jar, candle, mint plant, and mouse showed that plants restore to the air whatever breathing animals and burning candles remove.
Q106. No. of carbons in the primary CO2 fixation product of C4 plant is
Correct Answer: (c)
The primary product of CO2 fixation in C4 plants is Oxaloacetic acid (OAA), which is a 4-carbon organic acid.
Q107. The chemiosmotic mechanism mediates:
Correct Answer: (a)
Peter Mitchell's chemiosmotic theory explains how a proton gradient across the thylakoid membrane drives the synthesis of ATP.
Q108. To make 100 molecules of glucose, how many molecules of ATP & NADPH are required?
Correct Answer: (a)
Synthesis of 1 glucose molecule requires 18 ATP and 12 NADPH. Therefore, 100 glucose molecules require $100 imes 18 = 1800$ ATP and $100 imes 12 = 1200$ NADPH.
Q109. Consider the following statements:
(1) In C4 plants, the mesophyll cells lack RuBisCO enzyme.
(2) The basic pathway in the formation of the sugars is common to the C3 and C4 plants.
(3) The C4 plants lack a process called photorespiration.
Correct Answer: (d)
All three statements are fundamental characteristics of C4 plants and their highly efficient metabolic pathway.
Q110. All of the following statements are incorrect for non-cyclic electron transport system, except:
Correct Answer: (b)
Photolysis of 2H2O → 4H+ + 4e + O2. This oxidation happens in the Oxygen Evolving Complex associated with PSII.
Q111. Match the scientists given in column-1 with their work, given in column-II & select the correct answer using the codes given below.
Column-IColumn-II
A. PriestleyI. Determined the action spectrum of chlorophyll
B. Jan IngenhouszII. Provided evidence that in green parts of plant glucose is made & stored as starch
C. SachsIII. Plants purify air only in the presence of light
D. EngelmannIV. Demonstrated that photosynthesis is essentially a light dependent reaction
E. NielV. Revealed the essential role of air in the growth of plants
Correct Answer: (a)
Priestley (Air purification), Ingen-Housz (Sunlight requirement), Sachs (Glucose/Starch evidence), Engelmann (Action spectrum), Niel (Light dependent reaction/Hydrogen donor).
Q112. Which of the following pair is not correctly matched ?
Correct Answer: (a)
Maize is a C4 plant, not a C3 plant.
Q113. Which pigment acts directly to convert light energy to chemical energy ?
Correct Answer: (a)
Chlorophyll a is the only pigment capable of the primary photochemical event in the reaction center.
Q114. Which one of the following event occurs both during cyclic and non-cyclic modes of photophosphorylation?
Correct Answer: (b)
The common goal and result of both types of photophosphorylation is the synthesis of ATP using light energy.
Q115. Fixation of CO2 molecule through Hatch and Slack pathway requires an enzyme called _________ .
Correct Answer: (a)
The enzyme PEP carboxylase (PEPcase) performs the initial CO2 fixation in C4 plants.
Q116. Photorespiration:
Correct Answer: (a)
Photorespiration occurs when RuBisCO binds to O2 instead of CO2, leading to a wasteful process that releases CO2 and consumes energy.
Q117. Which of the following statement is incorrect?
Correct Answer: (b)
In the Z-scheme, electron movement is both uphill (when excited by light) and downhill (along the ETC carriers) in terms of the redox potential scale.
Q118. C4 plants are special because:
(i) they have a special type of leaf anatomy.
(ii) they tolerate higher temperatures.
(iii) they show a response to high light intensities.
(iv) they lack a process called photorespiration.
(v) they have greater productivity of biomass.
Correct Answer: (d)
C4 plants are highly efficient due to all these anatomical and physiological adaptations.
Q119. By looking at which internal structure of a plant can you tell whether a plant is C3 or C4?
Correct Answer: (a)
C4 plants exhibit a specialized leaf anatomy called Kranz anatomy, characterized by prominent bundle sheath cells arranged like a wreath around vascular bundles.
Q120. Chloroplast movement is influenced by
Correct Answer: (a)
Chloroplasts align themselves according to light intensity to either maximize absorption (low light) or avoid damage (high light).
Q121. The electrons that are released by the photolysis of water during non-cyclic photophosphorylation, ultimately end up in:
Correct Answer: (d)
In the non-cyclic pathway, electrons travel from water to PSII, then to PSI, and are finally accepted by NADP+ to form NADPH.
Q122. Which of the following statement is incorrect?
Correct Answer: (c)
NADPH formation is associated with Photosystem I, where electrons are transferred to NADP+ via ferredoxin.
Q123. Photosynthesis is the transformation of
Correct Answer: (a)
In photosynthesis, solar (light) energy is captured and converted into chemical energy stored in the bonds of glucose.
Q124. At low light intensities, the relation between the incident light and CO2 fixation rates is:
Correct Answer: (b)
At low light intensity, light is the limiting factor, and the rate of photosynthesis increases linearly with increasing light intensity.
Q125. In an experiment, the CO2 available to a C4 plant was labelled with a radioactive isotope and the amount of radioactivity in the chloroplast was measured. As photosynthesis preceeded, in which of the following molecules did the radioactivity first appear?
Correct Answer: (a)
In C4 plants, the first stable organic compound to incorporate radioactive carbon is the 4-carbon oxaloacetic acid.
Q126. Which of the following pair(s) is/are matched correctly?
Colour on the chromatogramPigment
(1) Blue greenChlorophyll a
(2) Yellow greenXanthophylls
(3) Yellow to yellow-orangeCarotenoids
Correct Answer: (a)
Chlorophyll a (Blue green) and Carotenoids (Yellow to yellow-orange) are matched correctly. Xanthophylls are typically just yellow.
Q127. In photosynthesis, protons accumulate in the:
Correct Answer: (c)
Protons are pumped from the stroma into the thylakoid lumen during electron transport, creating a high concentration (low pH) in the lumen.
Q128. In light reaction, plastoquinone facilitates the transfer of electrons from
Correct Answer: (d)
Plastoquinone (PQ) receives electrons from PSII and passes them to the Cytochrome b6f complex.
Q129. The given diagram represents the Calvin cycle.

At which stage (inducated by P, Q, R and S) is CO2 incorporated?
Correct Answer: (b)
CO2 is incorporated by reacting with RuBP (S) to form PGA (P) at stage Q (Carboxylation).
Q130. Site of photosynthesis in C4 plant is:
Correct Answer: (c)
In C4 plants, the reactions are split between mesophyll cells (initial fixation) and bundle sheath cells (Calvin cycle).
Q131. With reference to factors affecting the rate of photosynthesis, which of the following statements is not correct ?
Correct Answer: (b)
Statement (b) is incorrect because C4 plants actually have a higher temperature optimum than C3 plants.
Q132. Breakdown of water during the photosynthesis molecule leads to release of:
Correct Answer: (d)
Photolysis of water results in the release of oxygen, protons (hydrogen ions), and electrons.
Q133. Which of the following statement best support the fact that photorespiration commonly occurs in C3 plants?
Correct Answer: (d)
In C3 plants, RuBisCO is exposed to oxygen and functions as an oxygenase when CO2 concentration is low or O2 is high.
Q134. By which of the following complex, proton is pumped to reach ATP synthase, to participate in ATP synthesis?
Correct Answer: (a)
The cytochrome b6f complex uses energy from electron flow to pump protons from the stroma into the thylakoid lumen.
Q135. Which of following ratio is correct for the production of one molecule of glucose through 6 rounds of Calvin cycle?
CO2ATPNADPH2
(a)122
(b)61812
(c)61218
(d)569
Correct Answer: (b)
To produce 1 molecule of glucose (6C), 6 cycles are needed, consuming $6 imes 3 = 18$ ATP and $6 imes 2 = 12$ NADPH.
Q136. Which of the following is not a product of light reaction of photosynthesis?
Correct Answer: (b)
The light reaction produces NADPH (the reduced form of NADP+). NADH is a carrier used in cellular respiration, not photosynthesis.
Q137. How many molecules of RuBP & CO2 respectively are required for production of 6 molecules of 3-PGA?
Correct Answer: (c)
The reaction is: 3 RuBP (15 carbons) + 3 CO2 (3 carbons) → 6 PGA (18 carbons).
Q138. Which part of the plant do not perform photosynthesis?
Correct Answer: (d)
Typical epidermal cells of a leaf lack chloroplasts (except for guard cells) and therefore do not perform photosynthesis.
Q139. Contribution of Ingen-Housz in elucidation of process of photosynthesis is that:
Correct Answer: (a)
Jan Ingen-Housz showed that sunlight is essential for the process that purifies air and that only the green parts of the plant release oxygen.
Q140. Which of the following is used as an experimental material for making rapid advances in tracing path of carbon in Calvin cycle & other photosynthetic research ?
Correct Answer: (a)
Calvin used unicellular green algae like Chlorella and Scenedesmus in his radioactive carbon labeling experiments.
Q141. What will happen if the supply of oxygen is decreased to an illuminated wheat plant?
Correct Answer: (d)
Wheat is a C3 plant. Lowering oxygen concentration reduces photorespiration (competition for RuBisCO), thereby increasing the net rate of photosynthesis.
Q142. PEP is primary CO2 acceptor in:
Correct Answer: (a)
Phosphoenolpyruvate (PEP) is the initial CO2 acceptor in C4 plants, where it is converted into OAA.
Q143. In photosynthesis the light-independent reactions take place at
Correct Answer: (c)
The dark reactions occur in the fluid matrix of the chloroplast, the stroma.
Q144. Which of the following combination is correct for C4 plants?
Correct Answer: (a)
In C4 plants, initial CO2 fixation occurs in the mesophyll cells via PEPcase (forming the C4 cycle), while the final fixation via the Calvin cycle (C3 cycle) occurs in the bundle sheath cells using RuBisCO.
Q145. During the light stage of photosynthesis, the photoactivated pigment removes an electron from the hydroxyl radical derived from the water molecule. The fate of the free hydroxyl radical is that it:
Correct Answer: (a)
The splitting of water releases electrons, H+ ions, and oxygen gas as a byproduct.
Q146. Accessory pigments:
Correct Answer: (d)
By absorbing different wavelengths of light, accessory pigments allow the plant to use more of the visible spectrum for photosynthesis.
Q147. Emerson’s enhancement effect and Red drop have been instrumental in the discovery of
Correct Answer: (b)
Emerson showed that photosynthesis rate is higher when two wavelengths of light are given together, proving the existence of two linked photosystems.
Q148. In C4 plants, the process by which C4 acid is converted into C3 acid in the bundle sheath cell is known as
Correct Answer: (d)
C4 acids are decarboxylated in bundle sheath cells to release CO2 and a 3-carbon pyruvate.
Q149. Photosynthesis is important because:
(a) it is the primary source of food on earth.
(b) it is responsible for release of O2 into the atmosphere by green plants.
(c) it is responsible for release of water vapour into the atmosphere.
Correct Answer: (d)
Photosynthesis is the basis of all life on earth as the primary food source and it releases oxygen, which is essential for aerobic respiration.

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